Development of our tool was driven by real molecular-biology issues poisoning 454 and IonTorrent data

There is no other 454 adapter trimming tool looking for real issues.

Raw read trimming must reflect laboratory protocol and its artefacts

Adapters from some lab protocols occupy over 150nt of your precious reads.

We can at least rescue part of the deemed low-qual sequence to make the final sequence longer.

Detailed analysis reveals distribution of errors, artefacts, helps troubleshooting and tuneup.

Not always is the sequencer or vendor to be blamed.

Especially transcriptomic protocols leave by design adapters in high-qual sequence.

We can remove the offending adapters/MIDs/artefacts from your reads.

Once we do proper primer & adapter & MID removal assembly is possible and without artefacts!

Unremoved adapters prevent contig joining and scaffolding.

Increase your read lengths by proper analysis & trimming & trim point adjustments

Sometimes you could gain even 200nt of perfectly usable sequence.

Correlate sizes of DNA fragments before library preparation with insert sizes inferred from sequencing reads.

It is easy to compare BioAnalyzer traces with our charts.

Throw away reads with known technology-related artefacts

NextGen sequence data analysis & cleanup

In overall, minimize assembly artefacts, make the assembly feasible and correct.

We know what to look for in your raw data

We learned that from never-ending analyses of >1800 independent sample datasets

We can show how much sample sequence is buried in low-qual sequence while still usable.

Sometimes we can tell you based on the data what you did wrong.

Are you fiddling with base calling parameters of Roche software pipeline?

We can compare the results and tell you which was your best trial.

Titrate your libraries and sequence them. We then tell you which performed best.

We fix what others overlooked in the past.

We are fixing bugs still present in software from Roche and IonTorrent.